Alternatives to 35S as a label for the differential display of eukaryotic messenger RNA.
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چکیده
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Differential Expression of Arabidopsis thaliana Acid Phosphatases in Response to Abiotic Stresses
The objective of this research is to identify Arabidopsis thaliana genes encoding acid phosphatases induced by phosphate starvation. Multiple alignments of eukaryotic acid phosphatase amino acid sequences led to the classification of these proteins into four groups including purple acid phosphatases (PAPs). Specific primers were degenerated and designed based on conserved sequences of PAPs isol...
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1.Bauer, D., P. Warthoe, M. Rohde and M. Strauss. 1994. Detection and differential display of expressed genes by DDRT-PCR. PCR Methods Appl. 4:S97-S108. 2.Diatchenko, L.B., J. Ledesma, A.A. Chenchik and P.D. Siebert. 1996. Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA. Biochem. Biophys. Res. Commun. 219:824-828. 3.Liang, P. and A....
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Differential display has been developed as a tool to detect and characterize altered gene expression in eukaryotic cells. The basic principle is to systematically amplify messenger RNAs and then distribute their 3' termini on a denaturing polyacrylamide gel. Here we provide methodological details and examine in depth the specificity, sensitivity and reproducibility of the method. We show that t...
متن کاملDetection of eukaryotic cDNA in differential display is enhanced by the addition of E. coli RNA.
We describe a method to enhance the sensitivity of eukaryotic cDNA detection in differential display (DD). Typically, DD protocols require between 200 and 500 ng RNA for each reverse transcription reaction. The addition of Escherichia coli RNA before reverse transcription of eukaryotic RNA increases the detection of DD patterns more than tenfold. The method broadens the applicability of DD and ...
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Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...
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ورودعنوان ژورنال:
- Science
دوره 267 5201 شماره
صفحات -
تاریخ انتشار 1995